w v dss 0216011080 mp biomedicals  (Valiant Co Ltd)

Journal: iScience

Article Title: Enterohemorrhagic Escherichia coli senses colonic oxygen availability to modulate its virulence and colonization

doi: 10.1016/j.isci.2026.115632

Figure Lengend Snippet: Oxygen enhances T3SS-dependent EHEC colonization in vivo (A) Schematic of EHEC colonization in infection models. (B) CFUs recovered from the feces of mice infected with either EHEC O157:H7 WT or Δ escN (n = 8–9). (C) CFUs recovered from the colons of mice infected with EHEC O157:H7 WT (n = 8–9). Dextran sulfate sodium (DSS) can induce colitis, and 5-aminosalicylic acid (5-ASA) can restore epithelial hypoxia in mice. (D) Volcano plot illustrates the expression of differentially expressed genes ( P -adjust ≤ 0.01; |Fold Change| ≥2) in EHEC cultured under aerobic versus anaerobic conditions. The points in red are LEE-associated, and those in green are ArcA-associated. (E) KEGG pathways enrichment analysis performed on the downregulated DEGs from A. P -adjust ≤ 0.05. (F) KEGG pathways enrichment analysis illustrates the upregulated DEGs identified in A. P-adjust ≤0.05. (G) EHEC secreted proteins were analyzed using SDS-PAGE and western blotting (measured by anti-EspD antibody and anti-EspA antibody) following culture with or without oxygen. Results are representative of 3 biological replicate experiments with consistent results. (H) Transcription levels of ler were evaluated using quantitative real-time PCR (qRT-PCR) ( n = 7). ∗∗ p < 0.01 and ∗∗∗ p < 0.001, ns indicates no significant difference. Significance of differences was assessed using two-sided Mann-Whitney U-test (B and C) or two-tailed unpaired Student’s t test (H).

Article Snippet: Dextran sulfate sodium salt , Sangon Biotech , Cat#A461516.

Techniques: In Vivo, Infection, Expressing, Cell Culture, SDS Page, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, MANN-WHITNEY, Two Tailed Test